I can't seem to input the "IIS APPPOOL\PoolName" 'account' into the Windows file/folder ACL setting GUI, it doesn't recognise the account.
I understand that this was a problem in vanilla Windows 2008 and you needed to use icacls, but it's supposed to work on Windows 2008 R2 in the GUI.
See - http://forums.iis.net/t/1156692.aspx
Any ideas?
I had the same problem recently. After running IISRESET, I was able to assign permissions to the app pool identity accounts through the GUI as expected.