how to undo 'Alias' in Virtualhost

I wanted www.domain1.tld/domain2 to point to /www/directory2

So I added an 'Alias line' to my Virtualhost config apache2/sites-available/domain1.conf like this:

<VirtualHost *:80>  
    ServerName www.domain1.tld
    DocumentRoot /www/directory1

    Alias /domain2 /www/directory2    #Alias line
</VirtualHost>

Now I've removed that 'Alias line' and added a new Virtualhost config apache2/sites-available/domain2.conf where www.domain2.tld points to /www/directory2.

However, when I visit www.domain2.tld via a browser, the URL changes to www.domain1.tld/domain2 and I get a page saying "Not Found, The requested URL /domain2 was not found on this server"

How do I undo whatever the 'Alias line' did? It seems to have permanently modified some hosts - type file in my server.

(In case it matters, all this was in the service of a PrestaShop installation.)